摘要速递 | 卵泡液中的抗核抗体可能是体外受精和胚胎移植的危险因素

题目：

卵泡液中的抗核抗体可能是体外受精和胚胎移植的危险因素

期刊：

American journal of reproductive immunology (New York, N.Y. : 1989)

发表时间：

2022年 7月

关键词：

antinuclear antibodies; cell apoptosis; cell proliferation; cytotrophoblast; in vitro fertilization-embryo transfer.

中文翻译

目的

探讨抗核抗体(ANAs)对体外受精-胚胎移植(IVF-ET)结果的影响，并研究卵泡液中抗核抗体对胚胎发育的影响。

方法

将胚胎移植患者分为ANA阳性组和ANA阴性组。比较两组患者的年龄、不孕持续时间、体重指数(BMI)、基础促卵泡激素(bFSH)、抗苗勒管激素(AMH)、提取卵母细胞数、中期II期卵母细胞比例(MII)、卵裂期胚胎数、双核(2PN)、胚胎数、优质胚胎数及IVF-ET结局。体外培养HTR8/SVneo滋养细胞，分为ANA阴性组(对照组)和ANA阳性组(在细胞滋养细胞中加入anas阳性卵泡液)。采用ELSIA法检测体外受精-胚胎移植患者血清和卵泡液中的ANAs滴度。CCK-8法和流式细胞术(24h和48h)分别检测两组细胞的增殖和凋亡频率。

结果

在接受IVF-ET的患者中，卵泡液ANA阳性组的卵母细胞取出数、受精数和MII卵母细胞比例均显著低于卵泡液ANA阴性组。卵泡液ANA阳性患者着床率、临床妊娠率均低于阴性患者，早期流产率高于阴性患者。体外实验中，ANAs组细胞滋养细胞增殖活性明显低于对照组。此外，ANAs组细胞滋养细胞凋亡率显著高于对照组。

结论

我们的数据表明，卵泡液中的ANA可能通过促进滋养细胞凋亡和抑制滋养细胞增殖而成为胚胎发育的障碍。卵泡液中的抗核抗体可能是胚胎移植患者预后的不利因素。

英文原摘要

目的

To explore the effect of anti-nuclear antibodies (ANAs) on the outcome of in vitro fertilization-embryo transplantation (IVF-ET) and to study the effect of ANAs in follicular fluid (FF) on embryonic development.

方法

The expression of ANAs in FF of patients treated with IVF-ET and healthy group. The patients were divided into ANAs-positive group and ANA-negative group. The age, duration of infertility, body mass index (BMI), basic follicle stimulating hormone (bFSH), anti-Mullerian hormone (AMH), number of retrieved oocytes, portion of metaphase II oocytes (MII), number of embryos in the cleavage stage, bipronuclear (2PN), number of embryos, number of high-quality embryos and the outcome of IVF-ET were compared between the two groups. In vitro, HTR8/SVneo trophoblast cells were cultivated and divided into ANAs-negative group (control group) and ANAs-positive group (ANAs-positive FF was added to cytotrophoblasts). The ANAs titer in the serum and FF of patients who treated with IVF-ET was detected using ELSIA method. CCK-8 assay and flow cytometry (at 24 h and at 48 h) were used to detect the cell proliferation and apoptosis frequency of the two groups, respectively.

结果

Among those who underwent IVF-ET treatment, the number of retrieved oocytes, the number of fertilization and the portion of MII oocytes in the FF-positive group were significantly lower than those in the FF-negative group. Furthermore, the implantation rate and the clinical pregnancy rate were decreased, and early miscarriage rate was increased in the FF-positive patients than those in the FF-negative patients. In vitro, the cytotrophoblasts proliferation activity in the ANAs group was significantly lower than that in the control group. Moreover, the cytotrophoblasts apoptosis rate in the ANAs group was significantly higher than that in the control group.

结论

Our data suggested that ANAs in FF might become an obstacle to embryonic development through promoting trophoblast apoptosis and inhibiting trophoblast proliferation. ANAs in FF might be an unfavorable factor for the outcome of those who undergo IVF-ET treatment.

参考文献：

Wu S, Zhang L, Liu X, Jiang Y, Teng Y. Antinuclear antibodies in follicular fluid may be a risk factor in vitro fertilization and embryo transfer. Am J Reprod Immunol. 2022 Jul;88(1):e13560. doi: 10.1111/aji.13560. Epub 2022 May 11.