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J Dent:全酸蚀粘接剂中添加纳米铜对材料抗菌活性、机械性能以及树脂-牙本质界面粘接耐久性的影响

2017-04-28 lishiting MedSci原创

这篇研究是在酸蚀-冲洗(ER)后应用的粘接剂内添加不同浓度的纳米铜微粒,观察和评估材料的抗菌活性、Knoop显微硬度(KHN)、体外和原位转化率(DC)以及粘接即刻(IM)和2年(2Y)后树脂-牙本质的键合强度(μTBS)和纳米微渗漏(NL)变化。

这篇研究是在酸蚀-冲洗(ER)后应用的粘接剂内添加不同浓度的纳米铜微粒,观察和评估材料的抗菌活性、Knoop显微硬度(KHN)、体外和原位转化率(DC)以及粘接即刻(IM)和2年(2Y)后树脂-牙本质的键合强度(μTBS)和纳米微渗漏(NL)变化。

研究向粘接剂内添加不同比例的纳米铜(0 [control], 0.0075 to 1wt.%),以此获得7种不同规格的实验用含铜粘接剂。在粘接IM和2Y后,琼脂扩散法检测材料对变异链球菌的抗菌活性变化,同时检测粘接剂的Knoop显微硬度和体外DC改变。随后,使用实验用粘接剂应用于酸蚀后的咬合面牙本质。待树脂凝结后,纵向切开牙齿以获得束状树脂-牙本质样本(0.8 mm2)。在粘接IM和2Y后进行μTBS和纳米微渗漏的评估。粘接IM后,原位DC在束状树脂-牙本质样本内进行检测。实验数据进行相应的统计学分析(α=0.05)。

结果显示,添加了纳米铜的粘接剂仅在粘接即刻后表现出明显的抗菌活性,并且轻微减弱了材料的KHN、体外和原位的DC(纳米铜浓度:0.5 wt.%,或更少)。然而,在粘接2年后,所有浓度的纳米铜粘接剂其KHN均明显增强。与对照组相比较,2年后含铜粘接剂的μTBS减少(0.06 to 1% wt.%)和纳米微渗漏增加的变化均没有显著性差异。

结论:ER粘接剂中添加纳米铜微粒达到0.5 wt.%以上可以提高材料的抗菌活性,并且能够防止材料表面的降解,同时不会影响粘接剂的机械性能。

原始出处:

Gutiérrez MF, Malaquias P, et al. The role of copper nanoparticles in an etch-and-rinse adhesive on antimicrobial activity, mechanical properties and the durability of resin-dentine interfaces. J Dent. 2017 Apr 21. pii: S0300-5712(17)30091-X. doi: 10.1016/j.jdent.2017.04.007.

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  5. [GetPortalCommentsPageByObjectIdResponse(id=1804669, encodeId=8f991804669c4, content=<a href='/topic/show?id=b6b0e7473f0' target=_blank style='color:#2F92EE;'>#纳米铜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=50, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77473, encryptionId=b6b0e7473f0, topicName=纳米铜)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=hb2008ye, createdTime=Fri May 26 05:49:00 CST 2017, time=2017-05-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1985487, encodeId=8e95198548e4c, content=<a href='/topic/show?id=39e6803408a' target=_blank style='color:#2F92EE;'>#耐久性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80340, encryptionId=39e6803408a, topicName=耐久性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=48732500158, createdName=12498c32m68暂无昵称, createdTime=Tue Nov 07 21:49:00 CST 2017, time=2017-11-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1330569, encodeId=d6e0133056923, content=<a href='/topic/show?id=c6b455688d1' target=_blank style='color:#2F92EE;'>#抗菌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=76, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=55688, encryptionId=c6b455688d1, topicName=抗菌)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdqq.qlogo.cn/qqapp/1103841572/2898B09F0DA89357D0D54A725E611AC7/100, createdBy=fdd32500040, createdName=ms7899726347904398, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1441862, encodeId=be0914418625f, content=<a href='/topic/show?id=67526e55924' target=_blank style='color:#2F92EE;'>#牙本质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=58, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67559, encryptionId=67526e55924, topicName=牙本质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=37364903098, createdName=ms8538335253054589, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1538712, encodeId=705e1538e12e0, content=<a href='/topic/show?id=35fa55e03ee' target=_blank style='color:#2F92EE;'>#抗菌活性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=55703, encryptionId=35fa55e03ee, topicName=抗菌活性)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=fe6d13029843, createdName=xre2012, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1557139, encodeId=d8d5155e13901, content=<a href='/topic/show?id=18b8e6238e4' target=_blank style='color:#2F92EE;'>#粘接剂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=78, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76238, encryptionId=18b8e6238e4, topicName=粘接剂)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=32d114859562, createdName=qingting, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1627555, encodeId=a295162e555c2, content=<a href='/topic/show?id=cea46199eba' target=_blank style='color:#2F92EE;'>#树脂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61997, encryptionId=cea46199eba, topicName=树脂)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=378b21121654, createdName=chenshujs, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=)]
  6. [GetPortalCommentsPageByObjectIdResponse(id=1804669, encodeId=8f991804669c4, content=<a href='/topic/show?id=b6b0e7473f0' target=_blank style='color:#2F92EE;'>#纳米铜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=50, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77473, encryptionId=b6b0e7473f0, topicName=纳米铜)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=hb2008ye, createdTime=Fri May 26 05:49:00 CST 2017, time=2017-05-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1985487, encodeId=8e95198548e4c, content=<a href='/topic/show?id=39e6803408a' target=_blank style='color:#2F92EE;'>#耐久性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80340, encryptionId=39e6803408a, topicName=耐久性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=48732500158, createdName=12498c32m68暂无昵称, createdTime=Tue Nov 07 21:49:00 CST 2017, time=2017-11-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1330569, encodeId=d6e0133056923, content=<a href='/topic/show?id=c6b455688d1' target=_blank style='color:#2F92EE;'>#抗菌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=76, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=55688, encryptionId=c6b455688d1, topicName=抗菌)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdqq.qlogo.cn/qqapp/1103841572/2898B09F0DA89357D0D54A725E611AC7/100, createdBy=fdd32500040, createdName=ms7899726347904398, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1441862, encodeId=be0914418625f, content=<a href='/topic/show?id=67526e55924' target=_blank style='color:#2F92EE;'>#牙本质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=58, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67559, encryptionId=67526e55924, topicName=牙本质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=37364903098, createdName=ms8538335253054589, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1538712, encodeId=705e1538e12e0, content=<a href='/topic/show?id=35fa55e03ee' target=_blank style='color:#2F92EE;'>#抗菌活性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=55703, encryptionId=35fa55e03ee, topicName=抗菌活性)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=fe6d13029843, createdName=xre2012, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1557139, encodeId=d8d5155e13901, content=<a href='/topic/show?id=18b8e6238e4' target=_blank style='color:#2F92EE;'>#粘接剂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=78, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76238, encryptionId=18b8e6238e4, topicName=粘接剂)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=32d114859562, createdName=qingting, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1627555, encodeId=a295162e555c2, content=<a href='/topic/show?id=cea46199eba' target=_blank style='color:#2F92EE;'>#树脂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61997, encryptionId=cea46199eba, topicName=树脂)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=378b21121654, createdName=chenshujs, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=)]
  7. [GetPortalCommentsPageByObjectIdResponse(id=1804669, encodeId=8f991804669c4, content=<a href='/topic/show?id=b6b0e7473f0' target=_blank style='color:#2F92EE;'>#纳米铜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=50, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77473, encryptionId=b6b0e7473f0, topicName=纳米铜)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=hb2008ye, createdTime=Fri May 26 05:49:00 CST 2017, time=2017-05-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1985487, encodeId=8e95198548e4c, content=<a href='/topic/show?id=39e6803408a' target=_blank style='color:#2F92EE;'>#耐久性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80340, encryptionId=39e6803408a, topicName=耐久性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=48732500158, createdName=12498c32m68暂无昵称, createdTime=Tue Nov 07 21:49:00 CST 2017, time=2017-11-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1330569, encodeId=d6e0133056923, content=<a href='/topic/show?id=c6b455688d1' target=_blank style='color:#2F92EE;'>#抗菌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=76, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=55688, encryptionId=c6b455688d1, topicName=抗菌)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdqq.qlogo.cn/qqapp/1103841572/2898B09F0DA89357D0D54A725E611AC7/100, createdBy=fdd32500040, createdName=ms7899726347904398, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1441862, encodeId=be0914418625f, content=<a href='/topic/show?id=67526e55924' target=_blank style='color:#2F92EE;'>#牙本质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=58, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67559, encryptionId=67526e55924, topicName=牙本质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=37364903098, createdName=ms8538335253054589, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1538712, encodeId=705e1538e12e0, content=<a href='/topic/show?id=35fa55e03ee' target=_blank style='color:#2F92EE;'>#抗菌活性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=55703, encryptionId=35fa55e03ee, topicName=抗菌活性)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=fe6d13029843, createdName=xre2012, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1557139, encodeId=d8d5155e13901, content=<a href='/topic/show?id=18b8e6238e4' target=_blank style='color:#2F92EE;'>#粘接剂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=78, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76238, encryptionId=18b8e6238e4, topicName=粘接剂)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=32d114859562, createdName=qingting, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1627555, encodeId=a295162e555c2, content=<a href='/topic/show?id=cea46199eba' target=_blank style='color:#2F92EE;'>#树脂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61997, encryptionId=cea46199eba, topicName=树脂)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=378b21121654, createdName=chenshujs, createdTime=Sun Apr 30 00:49:00 CST 2017, time=2017-04-30, status=1, ipAttribution=)]
    2017-04-30 chenshujs
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