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Nat Aging:DNA甲基组揭示,人类单核细胞的健康衰老特征是什么?

2020-11-25 haibei MedSci原创

研究人员报告了人类经典单核细胞健康老化的全面特征,重点是表观基因组、转录组和蛋白质组的改变,以及相应的蛋白质组和血浆的代谢组数据。

即使在健康的人中,高龄会伴随着认知,代谢和生理能力的逐步下降,并可以提高神经退行性,血管和慢性炎症性疾病的易感性。目前,人们通常很难确定年龄相关的标志是否反映了单个细胞的变化或细胞类型丰度的变化,特别是在进行全组织转录或表观遗传学表征时。
尽管有大量的临床和流行病学数据,我们对健康个体的特定原代细胞群的年龄相关变化的性质了解甚少,特别是与年龄相关的表观遗传学景观的改变。

为了直接解决这个问题,研究人员专注于经典的CD14+CD16-单核细胞,因为它们是同质的,容易获得的,并且在血液中相对丰富,这允许研究人员从一次抽血中获得这些细胞的多组学剖析。
表观遗传学衰老可以表现在两个关键方面:年龄相关的染色质修饰变化和DNA甲基化变化。衰老和DNA甲基化之间的联系已经得到了很好的认可,然而,尽管有大量的研究,年龄相关的DNA甲基化/去甲基化的细胞特异性区域至今没有被报道。
在这里,研究人员报告了人类经典单核细胞健康老化的全面特征,重点是表观基因组、转录组和蛋白质组的改变,以及相应的蛋白质组和血浆的代谢组数据。该研究使用了20个年轻和20个老年男性的健康队列(平均年龄约27岁和约64岁)。

对于每个个体,研究人员进行了基于DNA甲基化谱的增强还原表征双亚硫酸盐测序,这使研究人员能够确定一组与年龄相关的差异甲基化区域(DMRs)--DNA甲基组中一个新的、细胞类型特异性的衰老特征。
低甲基化事件与低表达基因启动子附近CpG岛中的H3K27me3相关,而低甲基化DMRs则富集在H3K4me1标记的区域,并与相应基因表达的年龄相关增加相关。
因此,该研究提供了原代人细胞中DNA甲基化与年龄相关转录变化之间的联系。

原始出处:
Irina Shchukina et al. Enhanced epigenetic profiling of classical human monocytes reveals a specific signature of healthy aging in the DNA methylome. NATURE AGING 2020.

 

 

 

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    2021-01-18 liye789132251
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  3. [GetPortalCommentsPageByObjectIdResponse(id=1884811, encodeId=5791188481103, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Mon Jan 18 22:40:37 CST 2021, time=2021-01-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1299148, encodeId=e9b01299148e4, content=<a href='/topic/show?id=2b85362063c' target=_blank style='color:#2F92EE;'>#单核细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=36206, encryptionId=2b85362063c, topicName=单核细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=daa8281, createdName=雕雕, createdTime=Thu Nov 26 21:40:37 CST 2020, time=2020-11-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=902092, encodeId=9b2b90209210, content=低甲基化事件与低表达基因启动子附近CpG岛中的H3K27me3相关,而低甲基化DMRs则富集在H3K4me1标记的区域,并与相应基因表达的年龄相关增加相关。, beContent=null, objectType=article, channel=null, level=null, likeNumber=121, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20211019/df01a4bfb64246379ddfef3370d04115/e1274c8eb5e440918f56f83bcfea9031.jpg, createdBy=3a345397082, createdName=Jessica, createdTime=Wed Nov 25 10:47:56 CST 2020, time=2020-11-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=902070, encodeId=cb2b9020e022, content=该研究提供了原代人细胞中DNA甲基化与年龄相关转录变化之间的联系。, beContent=null, objectType=article, channel=null, level=null, likeNumber=107, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20200529/3611e9bb629c4e5b81fbd8ce784b06cc/128487eae357460bb98f67d86230c218.jpg, createdBy=ab235268530, createdName=神盾医疗局局长Jack, createdTime=Wed Nov 25 09:33:30 CST 2020, time=2020-11-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=902031, encodeId=6a829020310f, content=低甲基化DMRs则富集在H3K4me1标记的区域,并与相应基因表达的年龄相关增加相关。, beContent=null, objectType=article, channel=null, level=null, likeNumber=138, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20210325/2d9c955949df4b349a439f1a6773499e/b39f4845e869489d95bc545c79cf60fa.jpg, createdBy=303f5433779, createdName=Oranhyg, createdTime=Wed Nov 25 07:55:18 CST 2020, time=2020-11-25, status=1, ipAttribution=)]
    2020-11-25 Jessica

    低甲基化事件与低表达基因启动子附近CpG岛中的H3K27me3相关,而低甲基化DMRs则富集在H3K4me1标记的区域,并与相应基因表达的年龄相关增加相关。

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1884811, encodeId=5791188481103, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Mon Jan 18 22:40:37 CST 2021, time=2021-01-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1299148, encodeId=e9b01299148e4, content=<a href='/topic/show?id=2b85362063c' target=_blank style='color:#2F92EE;'>#单核细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=36206, encryptionId=2b85362063c, topicName=单核细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=daa8281, createdName=雕雕, createdTime=Thu Nov 26 21:40:37 CST 2020, time=2020-11-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=902092, encodeId=9b2b90209210, content=低甲基化事件与低表达基因启动子附近CpG岛中的H3K27me3相关,而低甲基化DMRs则富集在H3K4me1标记的区域,并与相应基因表达的年龄相关增加相关。, beContent=null, objectType=article, channel=null, level=null, likeNumber=121, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20211019/df01a4bfb64246379ddfef3370d04115/e1274c8eb5e440918f56f83bcfea9031.jpg, createdBy=3a345397082, createdName=Jessica, createdTime=Wed Nov 25 10:47:56 CST 2020, time=2020-11-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=902070, encodeId=cb2b9020e022, content=该研究提供了原代人细胞中DNA甲基化与年龄相关转录变化之间的联系。, beContent=null, objectType=article, channel=null, level=null, likeNumber=107, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20200529/3611e9bb629c4e5b81fbd8ce784b06cc/128487eae357460bb98f67d86230c218.jpg, createdBy=ab235268530, createdName=神盾医疗局局长Jack, createdTime=Wed Nov 25 09:33:30 CST 2020, time=2020-11-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=902031, encodeId=6a829020310f, content=低甲基化DMRs则富集在H3K4me1标记的区域,并与相应基因表达的年龄相关增加相关。, beContent=null, objectType=article, channel=null, level=null, likeNumber=138, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20210325/2d9c955949df4b349a439f1a6773499e/b39f4845e869489d95bc545c79cf60fa.jpg, createdBy=303f5433779, createdName=Oranhyg, createdTime=Wed Nov 25 07:55:18 CST 2020, time=2020-11-25, status=1, ipAttribution=)]
    2020-11-25 神盾医疗局局长Jack

    该研究提供了原代人细胞中DNA甲基化与年龄相关转录变化之间的联系。

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1884811, encodeId=5791188481103, content=<a href='/topic/show?id=3b2112532d8' target=_blank style='color:#2F92EE;'>#Nat#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12532, encryptionId=3b2112532d8, topicName=Nat)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2e6f107, createdName=liye789132251, createdTime=Mon Jan 18 22:40:37 CST 2021, time=2021-01-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1299148, encodeId=e9b01299148e4, content=<a href='/topic/show?id=2b85362063c' target=_blank style='color:#2F92EE;'>#单核细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=70, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=36206, encryptionId=2b85362063c, topicName=单核细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=daa8281, createdName=雕雕, createdTime=Thu Nov 26 21:40:37 CST 2020, time=2020-11-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=902092, encodeId=9b2b90209210, content=低甲基化事件与低表达基因启动子附近CpG岛中的H3K27me3相关,而低甲基化DMRs则富集在H3K4me1标记的区域,并与相应基因表达的年龄相关增加相关。, beContent=null, objectType=article, channel=null, level=null, likeNumber=121, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20211019/df01a4bfb64246379ddfef3370d04115/e1274c8eb5e440918f56f83bcfea9031.jpg, createdBy=3a345397082, createdName=Jessica, createdTime=Wed Nov 25 10:47:56 CST 2020, time=2020-11-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=902070, encodeId=cb2b9020e022, content=该研究提供了原代人细胞中DNA甲基化与年龄相关转录变化之间的联系。, beContent=null, objectType=article, channel=null, level=null, likeNumber=107, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20200529/3611e9bb629c4e5b81fbd8ce784b06cc/128487eae357460bb98f67d86230c218.jpg, createdBy=ab235268530, createdName=神盾医疗局局长Jack, createdTime=Wed Nov 25 09:33:30 CST 2020, time=2020-11-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=902031, encodeId=6a829020310f, content=低甲基化DMRs则富集在H3K4me1标记的区域,并与相应基因表达的年龄相关增加相关。, beContent=null, objectType=article, channel=null, level=null, likeNumber=138, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20210325/2d9c955949df4b349a439f1a6773499e/b39f4845e869489d95bc545c79cf60fa.jpg, createdBy=303f5433779, createdName=Oranhyg, createdTime=Wed Nov 25 07:55:18 CST 2020, time=2020-11-25, status=1, ipAttribution=)]
    2020-11-25 Oranhyg

    低甲基化DMRs则富集在H3K4me1标记的区域,并与相应基因表达的年龄相关增加相关。

    0

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众所周知,生活中经历的压力事件会让大脑提前衰老。近日,一项研究发现,基因突变与多种类型的精神压力相互作用。这些精神压力包括与细胞老化相关的创伤后应激障碍(PTSD)、疼痛和睡眠障碍。

NAT CELL BIO:肝脏细胞衰老分泌物促进肿瘤发生

研究人员发现,肝细胞特异性的果糖酶1,6-双磷酸酶1(FBP1)的缺失会破坏肝脏代谢平衡,促进肿瘤的进展。FBP1在人类和小鼠肝脏肿瘤中普遍存在沉默现象。

HUM REPROD:研究显示,生孩子会让女性的细胞衰老至少11岁

分娩不可避免地会给女性的身心带来巨大的变化,但做母亲的最终后果可能比我们想象的要大得多。一项对美国近2000名育龄妇女进行的DNA新分析显示,生过孩子的妇女遗传标记发生了改变,她们经历了明显加速的细胞衰老。

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