Exp Ther Med：含有多柔比星的阴离子，阳离子和中性脂质体处理后，人体牙龈衍生干细胞在2D培养中的细胞活力和成骨分化潜能

2019-02-04 MedSci MedSci原创

多柔比星，特别是阿霉素脂质体对干细胞的作用仍有待完全阐明。本研究旨在评估负载阿霉素的阴离子，阳离子和中性脂质体对二维培养中人牙龈衍生干细胞的活力和成骨分化潜能的影响。使用传统的薄脂膜水合方法制备负载阿霉素的脂质体。将干细胞接种在培养板上并保持在成骨培养基中。在倒置显微镜下观察干细胞的形态。使用细胞计数试剂盒-8测定法测定活细胞数。评估碱性磷酸酶活性并进行茜素红S染色以评估成骨分化。更高浓度的阿霉素

多柔比星，特别是阿霉素脂质体对干细胞的作用仍有待完全阐明。本研究旨在评估负载阿霉素的阴离子，阳离子和中性脂质体对二维培养中人牙龈衍生干细胞的活力和成骨分化潜能的影响。

使用传统的薄脂膜水合方法制备负载阿霉素的脂质体。将干细胞接种在培养板上并保持在成骨培养基中。在倒置显微镜下观察干细胞的形态。使用细胞计数试剂盒-8测定法测定活细胞数。评估碱性磷酸酶活性并进行茜素红S染色以评估成骨分化。更高浓度的阿霉素导致干细胞形态的明显变化。应用多柔比星后观察到细胞活力降低。多柔比星的应用，特别是在较高浓度下，碱性磷酸酶活性降低和茜素红S染色。

综上所述，本研究表明，在有或没有脂质体的情况下应用多柔比星会降低细胞活力和成骨分化。在不同的处理中，负载阿霉素的阳离子脂质体诱导干细胞培养物中细胞活力和成骨分化的最强降低。

原始出处：

Lee H, Son J, et al., Cellular viability and osteogenic differentiation potential of human gingiva-derived stem cells in 2D culture following treatment with anionic, cationic, and neutral liposomes containing doxorubicin. Exp Ther Med. 2018 Dec;16(6):4457-4462. doi: 10.3892/etm.2018.6777. Epub 2018 Sep 19.

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2019-05-17 shizhenshan

#脂质体#

61 0
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2019-10-20 HNYYM

#细胞活力#

59 0
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2019-06-30 HinsMax

#骨分化#

41 0
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2019-02-05 ms5880526374914242

#成骨分化#

74 0
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2019-02-05 zhaojie88

#成骨#

42 0
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2019-02-05 ms3994565386320060

#Med#

41 0
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2019-02-04 深海的鱼

学习学习学习

63 0
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2019-02-04 txqjm

谢谢了，学习

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