Baidu
map

INT J LAB HEMATOL:外周血涂片评估中的实时远程数字显微镜检查----观察者内部的一致性和经验

2019-02-01 MedSci MedSci原创

外周血涂片(PBS)审查是一项常规实验室检查,需要病理学家在发现异常指标,非典型细胞或发现关键问题时进行解释。当现场病理学家不可用时,实时远程数字显微镜(DM)可能有助于快速检查。在此,研究人员使用VisionTek M6机器人DM和TeamViewer成像软件评估PBS评估的观察者与光学显微镜(LM)和DM的一致性。 <span style="font-family:宋体;mso-asci

外周血涂片(PBS)审查是一项常规实验室检查,需要病理学家在发现异常指标,非典型细胞或发现关键问题时进行解释。当现场病理学家不可用时,实时远程数字显微镜(DM)可能有助于快速检查。在此,研究人员使用VisionTek M6机器人DMTeamViewer成像软件评估PBS评估的观察者与光学显微镜(LM)和DM的一致性。

由五名评价者评估了37个去识别标记的PBS载玻片。载玻片在异地实验室的VisionTek M6机器人显微镜上加载,并通过TeamViewer软件远程评估。评审员记录了解释,所需的时间(以分钟为单位),成像质量(0-3分)和解释置信度(0-3分)。此外,还记录了与DM评估相关的其他相关信息。随后在洗出间隔后使用LM评估载玻片。在DMLM之间比较观察者的印象,数字幻灯片质量,解释分钟和解释置信度的结果。

研究发现,LMDM之间的观察者内部一致性为93%,在每种评价模式下的135次评估中分别有9种不一致的解释。虽然审查人在DM模式下(5分钟/幻灯片)比LM模式(2.5分钟/幻灯片)花费更多时间,但评论者认为DM提供了足够的图像质量,并且评论者对幻灯片解释的置信水平在DM之间具有可比性(2.6 / 3 )和LM2.8 / 3)。

DMLM之间观察者内部一致性和可比较的解释置信度较高。DM可以作为非现场病理学家对PBS的评估的有用方法。

原始出处:

Diana M. Haninger Mehdi Nassiri Elizabeth D. Settembre. Live remote digital microscopy in peripheral blood smear evaluation: Intraobserver concordance and experience

本文系梅斯医学(MedSci)原创编译整理,转载需授权!


版权声明:
本网站所有内容来源注明为“梅斯医学”或“MedSci原创”的文字、图片和音视频资料,版权均属于梅斯医学所有。非经授权,任何媒体、网站或个人不得转载,授权转载时须注明来源为“梅斯医学”。其它来源的文章系转载文章,或“梅斯号”自媒体发布的文章,仅系出于传递更多信息之目的,本站仅负责审核内容合规,其内容不代表本站立场,本站不负责内容的准确性和版权。如果存在侵权、或不希望被转载的媒体或个人可与我们联系,我们将立即进行删除处理。
在此留言
评论区 (5)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=2078216, encodeId=b41620e821687, content=<a href='/topic/show?id=19498922824' target=_blank style='color:#2F92EE;'>#血涂片#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=89228, encryptionId=19498922824, topicName=血涂片)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a12645, createdName=yeye5224612, createdTime=Mon Feb 18 22:17:00 CST 2019, time=2019-02-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1906917, encodeId=88c8190691e2b, content=<a href='/topic/show?id=d91e1943441' target=_blank style='color:#2F92EE;'>#一致性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=19434, encryptionId=d91e1943441, topicName=一致性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3138208, createdName=zhanfl, createdTime=Mon Feb 11 02:17:00 CST 2019, time=2019-02-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1893787, encodeId=24d61893e8714, content=<a href='/topic/show?id=ab2159e343a' target=_blank style='color:#2F92EE;'>#显微镜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=59734, encryptionId=ab2159e343a, topicName=显微镜)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=54f9127, createdName=tulenzi, createdTime=Wed Jul 03 01:17:00 CST 2019, time=2019-07-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1317485, encodeId=22a5131e48519, content=<a href='/topic/show?id=74916e0414' target=_blank style='color:#2F92EE;'>#EMA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6704, encryptionId=74916e0414, topicName=EMA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a35a460, createdName=fengyi812, createdTime=Sun Feb 03 10:17:00 CST 2019, time=2019-02-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1578548, encodeId=8a2415e8548db, content=<a href='/topic/show?id=fd724282021' target=_blank style='color:#2F92EE;'>#外周血#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42820, encryptionId=fd724282021, topicName=外周血)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a31916414168, createdName=ms3994565386320060, createdTime=Sun Feb 03 10:17:00 CST 2019, time=2019-02-03, status=1, ipAttribution=)]
  2. [GetPortalCommentsPageByObjectIdResponse(id=2078216, encodeId=b41620e821687, content=<a href='/topic/show?id=19498922824' target=_blank style='color:#2F92EE;'>#血涂片#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=89228, encryptionId=19498922824, topicName=血涂片)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a12645, createdName=yeye5224612, createdTime=Mon Feb 18 22:17:00 CST 2019, time=2019-02-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1906917, encodeId=88c8190691e2b, content=<a href='/topic/show?id=d91e1943441' target=_blank style='color:#2F92EE;'>#一致性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=19434, encryptionId=d91e1943441, topicName=一致性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3138208, createdName=zhanfl, createdTime=Mon Feb 11 02:17:00 CST 2019, time=2019-02-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1893787, encodeId=24d61893e8714, content=<a href='/topic/show?id=ab2159e343a' target=_blank style='color:#2F92EE;'>#显微镜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=59734, encryptionId=ab2159e343a, topicName=显微镜)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=54f9127, createdName=tulenzi, createdTime=Wed Jul 03 01:17:00 CST 2019, time=2019-07-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1317485, encodeId=22a5131e48519, content=<a href='/topic/show?id=74916e0414' target=_blank style='color:#2F92EE;'>#EMA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6704, encryptionId=74916e0414, topicName=EMA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a35a460, createdName=fengyi812, createdTime=Sun Feb 03 10:17:00 CST 2019, time=2019-02-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1578548, encodeId=8a2415e8548db, content=<a href='/topic/show?id=fd724282021' target=_blank style='color:#2F92EE;'>#外周血#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42820, encryptionId=fd724282021, topicName=外周血)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a31916414168, createdName=ms3994565386320060, createdTime=Sun Feb 03 10:17:00 CST 2019, time=2019-02-03, status=1, ipAttribution=)]
    2019-02-11 zhanfl
  3. [GetPortalCommentsPageByObjectIdResponse(id=2078216, encodeId=b41620e821687, content=<a href='/topic/show?id=19498922824' target=_blank style='color:#2F92EE;'>#血涂片#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=89228, encryptionId=19498922824, topicName=血涂片)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a12645, createdName=yeye5224612, createdTime=Mon Feb 18 22:17:00 CST 2019, time=2019-02-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1906917, encodeId=88c8190691e2b, content=<a href='/topic/show?id=d91e1943441' target=_blank style='color:#2F92EE;'>#一致性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=19434, encryptionId=d91e1943441, topicName=一致性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3138208, createdName=zhanfl, createdTime=Mon Feb 11 02:17:00 CST 2019, time=2019-02-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1893787, encodeId=24d61893e8714, content=<a href='/topic/show?id=ab2159e343a' target=_blank style='color:#2F92EE;'>#显微镜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=59734, encryptionId=ab2159e343a, topicName=显微镜)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=54f9127, createdName=tulenzi, createdTime=Wed Jul 03 01:17:00 CST 2019, time=2019-07-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1317485, encodeId=22a5131e48519, content=<a href='/topic/show?id=74916e0414' target=_blank style='color:#2F92EE;'>#EMA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6704, encryptionId=74916e0414, topicName=EMA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a35a460, createdName=fengyi812, createdTime=Sun Feb 03 10:17:00 CST 2019, time=2019-02-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1578548, encodeId=8a2415e8548db, content=<a href='/topic/show?id=fd724282021' target=_blank style='color:#2F92EE;'>#外周血#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42820, encryptionId=fd724282021, topicName=外周血)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a31916414168, createdName=ms3994565386320060, createdTime=Sun Feb 03 10:17:00 CST 2019, time=2019-02-03, status=1, ipAttribution=)]
    2019-07-03 tulenzi
  4. [GetPortalCommentsPageByObjectIdResponse(id=2078216, encodeId=b41620e821687, content=<a href='/topic/show?id=19498922824' target=_blank style='color:#2F92EE;'>#血涂片#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=89228, encryptionId=19498922824, topicName=血涂片)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a12645, createdName=yeye5224612, createdTime=Mon Feb 18 22:17:00 CST 2019, time=2019-02-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1906917, encodeId=88c8190691e2b, content=<a href='/topic/show?id=d91e1943441' target=_blank style='color:#2F92EE;'>#一致性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=19434, encryptionId=d91e1943441, topicName=一致性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3138208, createdName=zhanfl, createdTime=Mon Feb 11 02:17:00 CST 2019, time=2019-02-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1893787, encodeId=24d61893e8714, content=<a href='/topic/show?id=ab2159e343a' target=_blank style='color:#2F92EE;'>#显微镜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=59734, encryptionId=ab2159e343a, topicName=显微镜)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=54f9127, createdName=tulenzi, createdTime=Wed Jul 03 01:17:00 CST 2019, time=2019-07-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1317485, encodeId=22a5131e48519, content=<a href='/topic/show?id=74916e0414' target=_blank style='color:#2F92EE;'>#EMA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6704, encryptionId=74916e0414, topicName=EMA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a35a460, createdName=fengyi812, createdTime=Sun Feb 03 10:17:00 CST 2019, time=2019-02-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1578548, encodeId=8a2415e8548db, content=<a href='/topic/show?id=fd724282021' target=_blank style='color:#2F92EE;'>#外周血#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42820, encryptionId=fd724282021, topicName=外周血)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a31916414168, createdName=ms3994565386320060, createdTime=Sun Feb 03 10:17:00 CST 2019, time=2019-02-03, status=1, ipAttribution=)]
    2019-02-03 fengyi812
  5. [GetPortalCommentsPageByObjectIdResponse(id=2078216, encodeId=b41620e821687, content=<a href='/topic/show?id=19498922824' target=_blank style='color:#2F92EE;'>#血涂片#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=72, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=89228, encryptionId=19498922824, topicName=血涂片)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a12645, createdName=yeye5224612, createdTime=Mon Feb 18 22:17:00 CST 2019, time=2019-02-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1906917, encodeId=88c8190691e2b, content=<a href='/topic/show?id=d91e1943441' target=_blank style='color:#2F92EE;'>#一致性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=61, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=19434, encryptionId=d91e1943441, topicName=一致性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3138208, createdName=zhanfl, createdTime=Mon Feb 11 02:17:00 CST 2019, time=2019-02-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1893787, encodeId=24d61893e8714, content=<a href='/topic/show?id=ab2159e343a' target=_blank style='color:#2F92EE;'>#显微镜#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=60, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=59734, encryptionId=ab2159e343a, topicName=显微镜)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=54f9127, createdName=tulenzi, createdTime=Wed Jul 03 01:17:00 CST 2019, time=2019-07-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1317485, encodeId=22a5131e48519, content=<a href='/topic/show?id=74916e0414' target=_blank style='color:#2F92EE;'>#EMA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=6704, encryptionId=74916e0414, topicName=EMA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a35a460, createdName=fengyi812, createdTime=Sun Feb 03 10:17:00 CST 2019, time=2019-02-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1578548, encodeId=8a2415e8548db, content=<a href='/topic/show?id=fd724282021' target=_blank style='color:#2F92EE;'>#外周血#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42820, encryptionId=fd724282021, topicName=外周血)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a31916414168, createdName=ms3994565386320060, createdTime=Sun Feb 03 10:17:00 CST 2019, time=2019-02-03, status=1, ipAttribution=)]

相关资讯

NEJM:间日疟原虫-案例报道

患者男性,24岁,来自于厄立特里亚因寻求庇护到达瑞士,近日因经常发热到急诊部就诊。  据该患者称,在过去的3年内,几乎每隔2个月,他就出现一次发热症状。  体格检查和常规血液检查发现轻度贫血和转氨酶水平升高。  快速抗原检测试验对疟疾呈阳性反应。  血涂片试验结果显示鞭毛生物的存在。最初怀疑患者疾病的发病与寄生虫感染有关,如微丝蚴、螺旋体或包柔氏螺旋

Baidu
map
Baidu
map
Baidu
map