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医学检验资格考试必背知识点--临床检验基础

2018-05-14 佚名 检验医学网

1、RBC计数液中硫酸钠用于提高相对比密防止粘连,氯化高汞是防腐剂。 2、HiCN法的缺点:KCN有毒,HbCO转化慢,高白血球和高球蛋白易混浊。 试验后处理应加入(先用水稀释,再用次氯酸钠混匀敞开放置15小时使CN氧化为N2和CO2或CO3和NH4。) 3、遗传性球形红细胞增多症:血象见许多小红细胞,且小红细胞生理性中心淡染区消失


临床检验基础部分

1、RBC计数液中硫酸钠用于提高相对比密防止粘连,氯化高汞是防腐剂。

2、HiCN法的缺点:KCN有毒,HbCO转化慢,高白血球和高球蛋白易混浊。

试验后处理应加入(先用水稀释,再用次氯酸钠混匀敞开放置15小时使CN氧化为N2和CO2或CO3和NH4。)

3、遗传性球形红细胞增多症:血象见许多小红细胞,且小红细胞生理性中心淡染区消失。

4、碱性点彩红细胞多见于铅等重金属毒,是铅中毒的筛选指标。

5、卡波氏环是胞质中脂蛋白变性所致,常与染色体小体同在,见于巨幼红和铅中毒。

6、LFR,MFR,HMR分别是低,中,高荧光率。

7、良性肿瘤ESR多正常,恶性肿瘤ESR多增加,低色素性贫血,遗传性球形红细胞增多症,镰状贫血时ESR减低。

8、杆状核过5%或有幼稚细胞为核左移,中性分叶核分叶5叶以上超3%为核右移。

9、钩虫病嗜酸细胞可达90%以上。嗜酸细胞白血病时亦可明显升高,但以幼稚为主。嗜酸细胞计数中乙醇或丙酮是保护嗜酸细胞而破坏其它,伊红是使嗜酸细胞着色,且1小时内应计数完成。

10、中性粒细胞毒性变化有:中毒颗粒,空泡,Doles小体,退行性变,大小不一。

11、异形淋巴细胞多为T淋巴细胞,分空泡、不规则、幼稚三型。

12、淋巴细胞有卫星核,即微核,是射线损伤后较为特殊的形态。

13、白血病时血小板(MPV)增高是骨髓造血恢复的第一征兆。

14、ABO遗传基因位于第9号染色体长臂三区四带,脑脊液中无血型物质,抗A与抗B主要是IgM,O型血中以IgG为主。

15、新生儿不宜做反向定型。

16、新生儿溶血的直接实验依据:红细胞直接抗人球蛋白阳性。

17、HLA分型的方法主要有:

(1)淋巴细胞毒试验,一类抗原用T或外周淋,二类抗原用B;

(2)混合淋巴细胞培养试验3HLA基因分析。

18、强直性脊椎炎有91%带有HLA-B27抗原而正常人只有6%。

19、血液保存液有:

(1)ACD(A枸橼酸C枸橼酸三钠D葡萄糖);

(2)CPD(C枸橼酸三钠P磷酸盐D葡萄糖腺嘌呤枸橼酸)。

20、枸橼酸是防止葡萄糖在消毒中焦化,腺嘌呤促进ATP合成延长红细胞寿命,磷酸盐提高PH。

21、输粒细胞时用ABO和Rh血型相同的血液,一般BPC小于二万时可输血。

22、糖尿病酮症:尿液呈烂苹果味,

泌尿系感染和膀胱癌:尿液呈腐败臭味,

苯丙酮尿症:尿液呈老鼠屎味。

23、尿液渗透浓度(OSM)是检查尿中可渗透溶质各质点实际浓度的一种指标,与粒子大小及电荷无关,主要取决于小分子物质的浓度(数量),是评价肾浓缩功能的良好指标。

24、晨尿的比密不超过1.018,或差值在0.001~0.002间,或恒定在1.010时说明肾浓缩稀释功能已基本消失。

25、低血钾碱中毒尿酸性高,高血钾酸中毒呈碱性尿,变形杆菌感染呈碱性,肾小管性酸中毒时尿PH值不低于6。

26、本周氏蛋白多见于多发性骨髓瘤,尿肌红蛋白多见于骨骼肌严重创伤大面积心梗。

27、选择性蛋白尿指数(SPI)即测定尿及血中IgG或白蛋白的比值来求得,大于0.2为选择性差,小于0.1为选择性好,尿中白蛋白/球蛋白大于5时为选择性蛋白尿。

28、80%饱和硫酸铵可沉淀其它蛋白而不能沉淀肌红蛋白可用来签别。

29、管型的基质蛋白为T-H蛋白。

30、肾静脉血栓形成时红细胞管型可能是唯一的表现。

31、尿PH(甲基红或溴麝香草酚),尿比密(多聚电解质:甲乙烯酸马来酐),尿蛋白(溴酚蓝),尿糖(色原:碘化钾,邻甲联苯胺),尿酮体(亚硝基铁氰化钠),尿胆红素(2,4二氯苯胺重氮盐),尿胆原(二甲氨基甲醛)尿亚硝酸盐(对氨基苯砷酸和1234四羟基对苯喹啉3酚)尿白细胞(吲哚酚酯)尿血红蛋白(过氧化氢茴香素或过氧化氢烯钴和色原)尿维C(中性红,缓冲剂,二氯酚靛酚钠)。

32、产后九天或人流25天后HCG恢复正常,产后四天人流十三天后HCG应小于1000,男性尿中HCG增高要考虑睾丸肿瘤。

33、镜检粪便中脂肪小滴大于6个/HP视为脂肪排泄过多。

34、正常粪便中G+球菌与G-杆菌比值约1:10。

35、隐孢子虫是AIDS和儿童腹泄重要病原诊断,且要查到卵囊。

36、正常空腹十二小时后胃液残余量为50ML。

37、脑脊液抽取时:第一管作细菌学检查,第二管作生化免疫学检查,第三管作细胞计数。

38、单纯疱疹脑炎时脑脊液中淋巴样细胞中可发现胞质内包涵体。

39、粒细胞、淋巴细胞、浆细胞同时存在是结核性脑膜炎的特点。

40、蛋白——细胞分离现象(Froin综合症)是蛛网膜下腔梗阻所致梗阻脑脊液的特征。

41、髓鞘碱性蛋白(MBP)增高见于多发性硬化症。

42、神经性梅毒检查首选试验是:密螺旋体荧光抗体吸收试验(FTA-ABC)。

43、浆膜积液(脑脊液)常规及细胞学检查用EDTA抗凝,生化检查用肝素。浆膜积液记得多留一管不加抗凝用于观察有无凝固。

44、漏出液以淋巴、间皮细胞为主,渗出液以中性粒、淋巴、嗜酸性细胞为多。

45、如见到纤毛柱状上皮和尘细胞可确认为痰液标本。

46、夏科—雷登结晶常与嗜酸细胞、库施曼螺旋体共存于大便中,见于支气管哮喘和肺吸虫病。

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    2018-05-30 wincls
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    2018-05-16 qblt
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    2018-05-15 惠映实验室

    学习了.谢谢分享.

    0

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    2018-05-14 惠映实验室

    学习了.谢谢分享.

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=2010018, encodeId=e415201001839, content=<a href='/topic/show?id=3a739256159' target=_blank style='color:#2F92EE;'>#资格考试#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92561, encryptionId=3a739256159, topicName=资格考试)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e0a1165, createdName=jiangfeng5067, createdTime=Wed Dec 26 08:02:00 CST 2018, time=2018-12-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2056523, encodeId=e3ef205652393, content=<a href='/topic/show?id=84f3e31660e' target=_blank style='color:#2F92EE;'>#知识点#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=67, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73166, encryptionId=84f3e31660e, topicName=知识点)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=991e83, createdName=wincls, createdTime=Wed May 30 08:02:00 CST 2018, time=2018-05-30, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1393344, encodeId=397513933449d, content=<a href='/topic/show?id=d64a8032596' target=_blank style='color:#2F92EE;'>#考试#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80325, encryptionId=d64a8032596, topicName=考试)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTKjqGDO7icVKUiaT7urgwXvnvzWtp0fMFU46Xfib8oavFKygiaWtibXReS6ZUh3PImsNicGAwTO5HXxoicnA/132, createdBy=ee262500130, createdName=ms7474195563676018, createdTime=Wed May 16 14:02:00 CST 2018, time=2018-05-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1509154, encodeId=3c06150915404, content=<a href='/topic/show?id=b1763415217' target=_blank style='color:#2F92EE;'>#医学检验#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=71, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=34152, encryptionId=b1763415217, topicName=医学检验)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=97f910060372, createdName=qblt, createdTime=Wed May 16 14:02:00 CST 2018, time=2018-05-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315466, encodeId=edfc315466d4, content=学习了.谢谢分享., beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/xkvRjYnJzzRJ2R0y7VhicibvkjPWHjIMmu4ZpKnE9cWAu4lVicS3uDUCfF4M4JWvrkrYfpb2KehPV0Ee29tPqyPEebBUibiaKhnvD/0, createdBy=f8412001184, createdName=惠映实验室, createdTime=Tue May 15 23:44:10 CST 2018, time=2018-05-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315146, encodeId=7c5e315146c9, content=学习了.谢谢分享., beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/xkvRjYnJzzRJ2R0y7VhicibvkjPWHjIMmu4ZpKnE9cWAu4lVicS3uDUCfF4M4JWvrkrYfpb2KehPV0Ee29tPqyPEebBUibiaKhnvD/0, createdBy=f8412001184, createdName=惠映实验室, createdTime=Mon May 14 23:41:59 CST 2018, time=2018-05-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315145, encodeId=2dec315145ff, content=学习了.谢谢分享., beContent=null, objectType=article, channel=null, level=null, likeNumber=102, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/xkvRjYnJzzRJ2R0y7VhicibvkjPWHjIMmu4ZpKnE9cWAu4lVicS3uDUCfF4M4JWvrkrYfpb2KehPV0Ee29tPqyPEebBUibiaKhnvD/0, createdBy=f8412001184, createdName=惠映实验室, createdTime=Mon May 14 23:41:52 CST 2018, time=2018-05-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315139, encodeId=b14b31513973, content=谢谢分享学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=134, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM4o2XVpJ6449yqDsjibydI5HnJ8MibGxNn4HZiaZib0bdfibbwoz3KQrIyN9AGxq36ZlZRNicYlPfOOWMtwgsajY6mGNFiaPclFdowjD0/0, createdBy=97bd1338592, createdName=清风拂面, createdTime=Mon May 14 23:40:29 CST 2018, time=2018-05-14, status=1, ipAttribution=)]
    2018-05-14 惠映实验室

    学习了.谢谢分享.

    0

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    2018-05-14 清风拂面

    谢谢分享学习

    0

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